Sentence examples for genes were digested from inspiring English sources
PCR amplifications of adc gene and ctfA_ctfB genes were digested by Xho I and Spe I restriction enzymes and cloned into pFC002 digested Xho I and Xba I to yield pFC005 and pFC006, respectively.
The PCR products of the motAB-his6 and motPS-his6 genes were digested with XmaI and SphI and cloned into the XmaI and SphI sites of an integration plasmid pDR67, yielding pDR-AB-His6 and pDR-PS-His6, respectively.
The PCR products containing the motAB and motPS genes were digested with BamHI and XmaI and cloned into the BamHI and XmaI sites of an expression plasmid pHT01, yielding pHT-AB and pHT-PS, respectively.
The amplified fragments of OsADF1, OsADF3 and GFP genes were digested with BamHI, XhoI or SpeI and cloned in-frame into the BamHI and SpeI restriction sites of the pubi::GFP vector to generate pubi::OsADF1-GFP and pubi::OsADF3-GFP.
The tags corresponding to each of the 20 specific genes were digested with restriction enzyme a and b.
10 µg of the selected plasmid for the genes were digested with 8 U of Hind III restriction enzyme overnight at 37°C.
Ribotyping is a similar method, by which 16S and 23S rRNA genes are digested with restriction endonucleases and Southern blotted using species-specific oligonucleotide probes.
An aliquot of each of the PCR-amplified 16S rRNA genes was digested at 37°C for 4 h and the resulting restriction fragments were separated on a 3% agarose electrophoresis gel.
The pHT01 plasmid and the amplified kIspS gene were digested using BamHI and XbaI restriction enzymes.
The fragment of SPT15 mutant gene and pYX212 plasmid were digested with EcoRI and SalI, however the pYX212 plasmid and TAF23 gene were digested by using restriction enzymes SalI/NheI and ligated by using T4 DNA Ligase (Thermo Scientific CO., LTD, Meridian, USA) to get pYX212-kan-SPT15-Mutant (pYX212-kan-SPT15-Mu) and pYX212-kan-TAF23-Mutant (pYX212-kan-TAF23-Mu) plasmids, respectively.
The pGEM-T Easy Vector containing YopP and the amplified kanamycin resistance gene were digested with SalI (NEB, Ipswich, MA) and SacI (Fermentas, Glen Burnie, MD) and ligated.
