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Polymorphisms on parasites pfcrt and pfmdr1 genes were determined with nested PCR followed by sequencing.
Haplotypes of 20 starch biosynthesizing genes were determined based on all tested polymorphic loci (markers) for each gene.
(C) Expression levels of the beiging-related genes were determined at indicated time points by the qPCR analysis.
The transcriptional start sites of the fadD1 and fadD2 genes were determined as previously described [30].
In the second step, the viral titer linked genes were determined.
Induction levels of the three T. thermophila genes were determined using quantitative real time RT-PCR.
Subsequently, full ORFs of these genes were determined using Artemis [30].
The functions of the predicted genes were determined using protein blast and Blast/CDD.
Positions of these genes were determined using the data files underlying NCBI's MapViewer browser (www.ncbi.nlm.nih.gov/mapview).nih.gov/mapview
ATR-responsive genes were determined with or without overexpression of SF-1.
Positions of tRNA-encoding genes were determined with tRNAscan-SE [61].
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