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Gene specific primers for candidate genes were designed using the beacon designer™.
Gene-specific primers for the identified genes were designed using the Beacon designer program.
The PCR primers for selected genes were designed using Primer350 with default parameters.
Cas9-target sites for the indicated genes were designed in http://crispr.genome-engineering.org.org
sgRNA sequences targeting specific genes were designed using publicly available tools37,66 or were derived from the Brunello library.
Specific primers for the grapevine PK genes were designed using the IDT PrimerQuest tool (https://www.idtdna.com/PrimerQuest/Home/Index).
A total of 105 probes selective for 42 genes were designed.
The sequences of Staphylococcus aureus and Escherichia coli genes were designed using software Primer Premier 5.0.
Probes for 16S ribosomal RNA and RNA polymerase β-subunit (rpoB) genes were designed.
Primers for the rice genes were designed to generate PCR products of 150 − 720 bp in length.
Primers for the amplification of GS and Gdh genes were designed based on the obtained consensus sequences (Table 1).
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