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Using Ingenuity Pathway Analysis IPAA) software, networks for >2 fold down-regulated epigenetic genes and up-regulated autophagy genes were created.
Relative standard curves describing the PCR efficiency of selected genes were created and used to perform efficiency-corrected quantification with the LightCycler Relative Quantification Software (Roche Molecular Biochemicals).
A series of plasmid constructs bearing partial sequences of the viral genes were created and each construct was used as a template for in vitro transcription.
Transgenic plants that expressed artificial microRNAs targeting these two genes were created.
Codon-modified E7 genes were created by site-directed mutagenesis of wild type E7 cloned into PUC 19 at EcoR1 and BamH1.
The chimeric class II genes were created by replacing exon 2 of the mouse gene with exon 2 of the corresponding human DR4 gene.
Similar(19)
Fusion genes are created by genetically fusing the open reading frames of two or more genes in-frame through ligation or overlap extension PCR.
Not all genes are created equal.
The entire concept of "silent" genes is created by our inability to detect extremely low transcript concentrations.
Tandemly arrayed genes are thought to be subject to birth-and-death evolution, in which new genes are created by duplication.
The control (null) set for the miRNA target genes was created using homology-based bioinformatics searches to identify appropriate paralog "pseudo-target" genes from Arabidopsis.
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