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Based on the co-expression patterns, putative regulatory networks between TFs and SCW-associated genes were constructed.
Furthermore, putative regulatory networks involving TFs and SCW-associated genes were constructed based on gene co-expression patterns.
Primer and probe designs for these resistance genes were constructed and optimised for application in standardised TaqMan PCR assays.
Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection.
Synechocystis sp. PCC 6803 strains overexpressing pha genes were constructed and characterized for poly-3-hydroxybutyrate (PHB) production.
Two strains, each targeting one of the top model-identified genes, were constructed and tested in vivo.
Different overexpression vectors harboring combinations of tHMG1, IDI, ERG20 and codon-optimized α-farnesene synthase (OptFS) genes were constructed and integrated into the genome of Y. lipolytica Po1h.
Plasmids for markerless deletion of CA genes were constructed according to Lu et al. [11].
To assess the promoter activities of several promoters in B. subtilis GRSW2-B1, pHY300PLK derivatives containing the promoter::lacZ transcriptional fusion genes were constructed (Table 1).
Individual GST-fusions of Dm-AMP1 and Rs-AFP2 genes were constructed in pEG KT) vector (Mitchell et al. 1993) and expressed in Saccharomyces cerevisiae strain, s288C.
Yeast strains overexpressing the target genes were constructed by replacing the promoter region of each gene with that of TDH3 gene, which encodes glyceraldehyde-3-phosphate dehydrogenase (GPDH).
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