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Finally, six genes were confirmed to be up-regulated by hypergravity.
The DNA sequences of the chemically synthesized DHQase genes were confirmed by DNA sequencing.
Expression levels of identified genes were confirmed by real-time RT-PCR.
The integrated genes were confirmed by Southern blot and 1,2-PD production was analyzed by HPLC.
Selected genes were confirmed by RT-PCR.
Deletions of all genes were confirmed by PCR and sequencing analysis.
In this analysis 25 of the 26 tested genes were confirmed as differentially expressed (Table 1).
The nucleotide sequences for the cloned genes were confirmed by sequence analysis.
Changes in mRNA levels of several of these genes were confirmed by qRT-PCR (Table 2).
Dysregulated genes were confirmed in SM tissue and SMCs from the smDicer KO mice through qPCR.
Cloned genes were confirmed by restriction analysis and by DNA sequencing of both strands.
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