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The complete xopAF and avrGf1 genes were complemented in the single and double mutants using pUFR053 and pUFR034 respectively.
Knockout mutants of the fur (previously constructed [ 24]), perR, katA, ahpC, and sodB genes were complemented by insertion of the wild-type gene into the chromosome following the methodology developed by Karlyshev et al. [ 29], using either the pRR-Km [ 58] or pRR-Cm plasmid [ 29].
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This ability to precisely perturb essential genes is complemented by the inherently highly reproducible nature of the zygotic division that facilitates development of quantitative imaging assays.
These deleted genes are complemented by other genes (iolE1, iolE3, iol and iolG1).
This study of the whitefly Bemisia tabaci and two bacteria (Portiera aleyrodidarum and Hamiltonella defensa) cohoused in each host cell investigated whether the decay of Portiera metabolism genes is complemented by host and Hamiltonella genes, and compared the metabolic traits of the whitefly symbiosis with other sap-feeding insects (aphids, psyllids, and mealybugs).
Interestingly, the two introduced R genes are complementing the resistance spectrum that was already present in Potae9 plants.
Since these gene classes are particularly redundant in B. fragilis, it is reasonable to presume that the deleted gene is complemented by homologous members.
Presumably, absence of the gene is complemented by corresponding enzymes either from Ishikawaella itself or from the host insect (supplementary table S5, Supplementary Material online).
We generated S. cerevisiae "factor swap" strains in which deletion of an endogenous gene was complemented with the K. lactis ortholog, and we performed MNase-Seq to map nucleosomes in these strains.
In cases of gene redundancy, a disrupted gene may be complemented by another gene (presumably a homolog), and it would be expected that genes with multiple homologs might not be essential for survival of B. fragilis 638R but will result in synthetic lethality if all homologs are targeted.
This work clearly demonstrates that analyses of distribution patterns of genes should be complemented with phylogenetic reconstructions of the genes themselves in order to distinguish between differential gene loss and gene transfer [ 19].
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