Assessment of genetic diversity based on genome-wide SSRs and locus-specific candidate genes are complementary investigations, the former providing a global view of the rye genome and the latter restricted to genes involved in the frost tolerance network.
The primer CS1d (16 ), which is used traditionally to amplify the full-length gltA gene, is complementary to the ultimate 5′-end of the open reading frame for this gene of R. prowazekii.
Neither EcoRI nor BamHI cut within the region of the opsin genes that were complementary to the probes.
A positive correlation was found in gene expression between protein-coding genes and the homologous mlncRNAs in response to cold and dehydration stresses, while the correlation was negative when protein-coding genes and mlncRNAs were complementary to each other.
In the other duplication sets, the expression patterns of both member genes were partial complementary and/or overlapped.
In order to create scFv fragment genes as a VH-linker-VL type, the 3' ends of VH genes were made complementary to 5' ends of VL genes through a (Gly4Ser)3 linker peptide, and the VH and VL genes were assembled and amplified by overlap extension followed by pull-through PCR.
Notably, the patterns of expression of the two genes under oxidative stress were complementary to the patterns seen under osmotic stress with glucose or NaCl.
Reads mapping to one gene in a pair were complementary to a corresponding region of the other gene.
