Mutant strains lacking sortase genes are attenuated in virulence in animal models of several bacterial infections [3], [4], [5], leading to the recognition of sortase enzymes as promising new therapeutic targets [6], [7].
As shown in Figure 4A, under high 25OH-cholesterol conditions, a majority of the genes identified as activators in our screen were clustered in the bottom left corner of the scatter plot indicating that the activity of these genes was attenuated in the presence of excess cholesterol.
Although expression induction of these genes was attenuated at 96 hrs, significant expression was still detectable.
In livers from GLP-1 oestrogen-treated mice, expression of lipoGLP-1 oestrogen-treatedted and hepatic triacylGLP-1 oestrogen-treatedcreased.
In addition, it has been shown that the differential expression of auxin-induced genes is attenuated within 3 h [ 8].
Furthermore, in trypanosomes an array of genes is attenuated, whereas in the olfactory system only the OR is silenced.
Consequently, the negative effect of downregulated miRNAs on their target genes is attenuated, which leads to the activation of the transcription factor genes SOX9, MEF2C, TRPS1 and SATB2.
During the process of neural differentiation, the repression of neuroectodermal genes is attenuated, which is accompanied by an increase in acetylation of target genes.
The transcriptional induction of these detoxifying and antioxidant genes is attenuated when NRF2 is knocked down, indicating that the induction is mediated by NRF2.
This complex is a virulence determinant for Helicobacter pylori, Mycobacterium tuberculosis, Actinobacillus pleuropneumoniae and S. enterica, as mutants on these genes are attenuated [ 40- 43].
