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Shendure slices up the genome and runs it over a microscope slide where known portions of the protein genes are attached.
The team then modified the bacterial genome so that the regulatory DNA responsible for activating one of these genes was attached to a gene for a protein that glows when produced.
In short, a microarray is a physical support to which oligonucleotides (the probes) representing the genes are attached.
We extracted several relations the score value of which was greater than 0.2, and the list of selected genes was attached as Supplementary File 2. Based on our evaluation system (Supplementary File 1), we extracted an artificial gene network involving 3 genes: SPARC, NLR family, pyrin domain containing 12 (Nankyrin ankyrin 1 (ANK1).
After network topology construction, the m E-genes were attached uniform randomly over all S-genes.
Similarly, the 14C5 VL domain gene was attached to the CL of a human IgG1 light-chain gene.
A biotinylated trypsinogen gene is attached to streptavidin-functionalized magnetic beads.
It is assumed that each E-gene is attached to a single S-gene only (Fig. 1).
We also tested the artificial constructs of osa gene in which the gene was attached to a constitutive activating or repressor domain (Collins et al. 1999).
Nuclear matrices prepared with 2 m NaCl were primarily associated with intergenic gene-poor regions and genes that were attached to the nuclear matrix were silent.
Cub and receptor gene LexA-VP16 were attached at the C-terminus of cdh23, i.e., intracellularly.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com