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Notably, Group IV SERA genes were ascertained in all mammalian parasite lineages; and in two primate parasite lineages gene events such as duplication, truncation, fragmentation and gene loss occurred at high frequency in a manner that mimics the birth-and-death evolution model.
Polymorphisms in three glutathione S-transferases genes were ascertained using TaqMan Polymerase Chain Reaction (PCR) allelic discrimination assays.
For genotype interaction analysis, all possible genotype combinations between selected SNPs (pairwise or multiple genes) were ascertained from a MassArray platform for the given genotypes of SNPs.
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Chromosomal distribution of IR genes was ascertained and each individual gene of IR gene families was also mapped on the chromosomal arms of An. gambiae.
The significance of pairwise F ST values for the COI and the EF-1α genes was ascertained by 1000 permutations.
Finally, (iv) the inheritance of the unaffected or affected genes was ascertained by examining the parental haplotypes in the region of interest, including the gene locus, in comparison with the reference.
For instance, a figure of 18.6% of species-specific genes was ascertained for Drosophila melanogaster in a genome comparison which included Anopheles gambiae as the other insect representative [ 8].
The functional significance of all genes was ascertained based on several online databases (Genecards - http://www.genecards.org/, OMIM - http://www.omim.org, NCBI - http://www.ncbi.nlm.nih.gov, BioGraph - http://biograph.be).
To address this question the skeletal muscle specificity (SPi: see Materials and Methods: Data Input to the Virtual Muscle) of the expression of each of the genes was ascertained by interrogating the SymAtlas mouse GNF1M dataset which contains a tissue survey of mouse gene expression (see Additional File 1).
Optimal PCR conditions for each gene were ascertained, guaranteeing that generation of the PCR-products was in the exponential phase.
Moreover, the specificity of the siRNA knockdown of the CD49e gene was ascertained by the fact that no effect was seen with a scramble siRNA.
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