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There is genetic variation within other genes we did not evaluate and the selected genes we studied in the study.
To assess the impact of DNA methylation on genes, we studied orthologous genes of two sea squirt species and two fish species.
It seems that allele sharing and retention of ancestral polymorphisms is present only at a very low level, at least for the four genes we studied.
This is why we have taken an expression cut-off of genes that are 1.3 fold up-regulated and above, although many of the genes we studied were very highly differentially regulated.
Exceptionally high level of germ line SNP mutations in RASSF1A found in several studies [8] support our data that the two genes we studied have rather frequent mutations even in normal cells.
The second group of genes we studied consisted of the neuronal cytoskeletal protein NFH- the heavy subunit of neurofilaments (NF)- and GAP-43, which is a phosphoprotein associated with axonal growth cones and expressed at high levels by neurons during axon growth [38]; moreover we assessed the mRNA expression of GFAP, a protein of the cytoskeleton of astrocytes.
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GUARENTE: The genes we study counteract aging.
As our understanding of these processes and the group of genes we study increases in sophistication, it becomes increasingly important to account for multiple components of a mutant phenotype rather than a single aspect (such as cell number).
Taking into account the determinant role of the promoter region in the expression of a gene, we studied the relationship between positive selection and gene expression.
In order to further address the relationship of the Y/F/WxC-genes, we studied their intron structure revealed by comparison of the EST sequences and the genomic data available on http://www.ncbi.nlm.nih.gov.nih.gov
In the PPARG gene, we studied the Pro12Ala polymorphisms, and in TNFA gene, the Gly318Ala polymorphism was studied.
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