Of these genes, we detected no signal for the ACO1 and ACO5 transcripts, and the transcript levels of ACO2 showed a decreasing pattern over time (data not shown).
Among the increased 'TSA sensitive' genes we detected several transcription factors including Id2, Egr1, and Sox11, whose down-regulation is critical for OPC differentiation.
In order to determine the impact of CGI methylation on the expression of associated genes, we detected mRNA levels of a subset of genes via qRT-PCR.
In addition to the expected omcA and omcB genes, we detected five adjacent genes sharing a similar N-terminus and conserved cysteine residues, which may form an extended omc family, both in W. chondrophila and P. amoebophila.
Among the 18 ADH genes we detected in CH34, the most likely candidate for an isopropanol dehydrogenase (iPDH) is Rmet_5645 because its product is highly similar in length and sequence to the multifunctional ADH of C. eutrophus H16 (H16_A0757) [194].
Testing whether the higher degree of binding of p53 phosphorylated at S46 is correlated with an expression change of the respective target genes, we detected for BCL2L1, FAM46A, and TGFA a statistically significant difference in expression change for Etoposide versus Actinomycin D treatment (Figure 6B).
For nodulation genes, we detected footprints of selection in two genes DMI1 and NIN.
Therefore, certain genes we detected may be age-related rather disease-specific.
For 19 genes, we detected transcripts under Fe minus or/Cu minus conditions.
For 22 of the genes, we detected PCR products exclusively from conjugating cells (Fig 1B, marked with yellow).
In contrast to the amplification of VEGF pathway genes, we detected significant over-representation of deleted genes in 11 pathways.
