Sentence examples for genes we designed from inspiring English sources
For Rh genes, we designed 10 primer sets to amplify all 10 exons.
To determine the reprogramming potential of germ cell- specific genes, we designed a polycistronic gene cassette expressing Stella, Oct4 and Nanos2 in a lentiviral-based vector.
To identify novel, potentially pathogenic variants in additional genes, we designed a panel of genes identified from studies on familial forms of isolated or syndromic CAKUT and genes suggested by in vitro and in vivo CAKUT models.
To clone the genes, we designed primer pairs to amplify each open reading frame by PCR and inserted them individually into pXDC61M to generate translational fusions with the upstream β-lactamase gene (Fig. 1A).
To minimize the possibility that genetic modification of the Rtn4r locus could affect expression of the two neighboring genes, we designed and implemented a gene targeting strategy (Fig. 2B F) to replace exon II of Rtn4r with the self-excisable selection cassette that includes the neo gene selectable marker (pACN) [40].
In order to obtain even greater ORF coverage of ABP genes, we designed genome walking primers and amplified 5' and/or 3' ends of PAL, CHS, CHI, F3H, DFR and ANS using the manufacturer's suggested protocol (GenomeWalker Universal Kit, Clontech Laboratories, Mountain View, CA).
To improve sheep reproduction through modification of the BMPR-IB gene, we designed an sgRNA to target the sheep BMPR-IB gene by using the CRISPR/Cas9 system.
To investigate any copy number variations in this enormous gene, we designed a novel custom comparative genomic hybridization microarray, NM-CGH, targeted towards the seven known genes causative for NM.
To deal with possible variability in our results due to the white gene, we designed genetic controls that had the same w1118 mutation and the same numbers of mini-w transgenes, differing therefore only in their genetic backgrounds.
To determine the genetic environment of the blaCTX-M-15 gene, we designed an inverse PCR.
To facilitate the genotyping of Arg462Gln and Asp541Glu polymorphisms of the RNASEL gene, we designed primers covering these polymorphic sites.
