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The mRNA abundance of several appetite-associated neuropeptide genes was quantified and hypothalamic neuropeptide Y (NPY) mRNA was increased in PrRP-injected chicks.
Shoots were sampled at the F3 (tillering), F7 (jointing), F10 (heading) and F11 (grain filling) stages, and expression of the five genes was quantified using real-time, reverse transcription PCR (polymerase chain reaction).
In order to investigate the difference between submergence tolerance as mediated by the strong allele SUB1A-1 (present in IR64-Sub1) and the weak allele SUB1A-2 (present in IR64), expression of 2487 rice TF genes was quantified by qPCR in internodes of submerged and non-submerged control plants.
First, the mRNA level of selected genes was quantified using real time RT-PCR (qRT-PCR) (Figure S5).
Expression of mRNA for individual genes was quantified by real-time PCR using an ABI Prism7500 DNA analyzer (Applied Biosystems) as described previously [42].
The mRNA of the remaining 26 genes was quantified in the ex vivo material from the nose and compared to the transcription of the nose isolates during growth in vitro.
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Abundances of transcripts of target genes were quantified by quantitative real-time reverse transcription PCR (qPCR).
In addition to expression of cyp1a, abundances of transcripts of several other genes were quantified.
Normalised expressions of the AmSesTPS1 and AmGuaiS1 genes to the housekeeping genes were quantified in the 54 samples using the same batch of cDNAs to minimise experimental variation.
The genes were quantified in triplicate.
Genes were quantified using only the single-best mapping methodology.
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