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Knock-down efficiency of shRNAs on target genes was determined by qPCR.
HepG2 cells were treated with GN and the expression of JMJD2B, PPARγ2, and its steatosis genes was determined by qPCR.
Transcript abundance of candidate hexose transporter genes was determined using reverse transcription quantitative polymerase chain reaction.
The chromosomal localization of human and murine TAPA-1 genes was determined by Southern blot experiments using DNA from somatic cell hybrids.
The expression of these genes was determined by quantitative real-time polymerase chain reaction (qRT-PCR) in females of Kisumu, unexposed MRS and MRS insecticide survivors.
The germline configuration and the exon intron organization of the 8 TRGC genes was determined, six of them resulting functional.
Expression of the coffee carotenoid genes was determined in leaf, branch and flower tissues using quantitative RT-PCR.
METHODS: The expression of pro-angiogenic genes was determined by quantitative real-time RT-PCR.
The transcript abundance of the reference genes was determined by the Ct value.
b The expression of OsSMF1 and its target genes was determined by RT-PCR analysis.
The spatial distribution of all 30 motifs in the promoter regions of the predicted genes was determined (Fig. 1).
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