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"Late pollen-preferred genes" was defined as preferentially expressed genes at pollen following the bi-cellular stage.
The significance of the differential expression of genes was defined by the bioinformatics service of BGI according to the combination of the absolute value of log2-Ratio ≥1 and FDR ≤ 0.001.
This group of genes was defined as Pezizo-specific genes.
This subset of 146 genes was defined as the common signature of ependymoma recurrences (Table S5).
The set of AU-rich genes was defined based on the presence in ARED database 3.0 [3.0.
As some TCSTS gene pairs are adjacent to the genes that they control, the genetic structure of the salKR locus and its flanking genes was defined (Fig. 2A).
For this analysis, the set of modulated genes was defined as the set of genes with a fold induction greater than 1.5 in both experiments.
Each gene loci is a range of base pairs and the distance between two genes was defined as the head to tail distance except when a miRNA is in the intron of an mRNA.
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Early- and late-replicating genes were defined by calculating the average WA score over the promoter.
The remaining genes were defined as 'non-expressed genes'.
Differentially expressed genes were defined as those having P<0.05 compared with WT-untreated mice.
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