Sentence examples for genes was checked using from inspiring English sources

The annotation of tRNA genes was checked using tRNAscan version 1.21 [ 51].

The specificity of each primer to their target genes was checked using the BLASTN program of the cucumber genomic database.

The specificity of each primer to their target genes was checked using the BLASTN program of BRAD.

Probable paralogous gene family members of all candidate genes were checked using the information of all rice paralogous gene family members [66].

Specificity of primer pairs (Additional file 5: Table S3) for each gene was checked using NCBI Primer BLAST against the nr database.

Eggs electroporated with cytoplasmic Cystatin, β-Actin-dsRNA, buffer and irrelevant lacZ-dsRNA and the endogenous expression of target gene was checked using real time RT-PCR one week following electroporation.

Similarity across genomes for TEs predicted by the REPET TEdenovo pipeline (excluding incomplete and potential host gene sequences) was checked using hierarchical clustering with the h-cd-hit-est program from the CD-HIT Suite Server (http://weizhongli-lab.org/cd-hit/ 72.

After incubation, total RNA was extracted from all samples and gene silencing was checked using RT-PCR.

The quality of the 16S rRNA gene sequences was checked using the multiple alignment CLUSTALW software package [ 37].

To avoid unintentional silencing of non-targeted host cell genes, sequence homology was checked using a basic local alignment search tool (BLAST) search (http://blast.ncbi.nlm.nih.gov/Blast.cgi).nih.gov/Blast.cgi

Results on differentially expressed genes were compared to 5 major expression studies available from the PubMed GEO project [ 14- 18] and evidence of independent validation of gene expression data was checked using the PubMed database.