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The relative expression level of each genes was calculated using the 2−ΔΔCt method.
To calculate the enrichment of CD45+ cells after MACS Cell Sorting, the mean signal intensity of the five platelet specific genes was calculated for each sample.
Expression of the starch-synthetic genes was calculated as a relative ratio to the geometric mean of the four internal standard genes (Vandesompele et al. 2002).
Statistical significance of enrichment of environmental stress genes was calculated through the Fisher's Exact Test.
Relative expression of target genes was calculated using the comparative threshold cycle method.
The average intensity of the 41 ubiquitous genes was calculated for each reference array.
Relative expression of the analyzed genes was calculated according to manufacturer's instructions.
Relative expression of target genes was calculated using standard curve method and normalized to HPRT mRNA content.
The number of active LMW-GS genes was calculated for each of the eight genotypes (Table 3).
Relative expression of target genes was calculated as 2−ΔΔCT.
A hierarchical cluster analysis of these 9000 genes was calculated.
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