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Markerless insertion and/or deletion of genes was achieved by establishing counter-selective markers, which are used to remove "unwanted" DNA from the chromosome (Pritchett et al. 2004; Moore and Leigh 2005).
Results obtained with RNA trans-splicing molecules designed to target the 3′ end of mouse titin and human dysferlin pre-mRNA transcripts indicated that trans-splicing of pre-mRNA generated from minigene constructs or from the endogenous genes was achieved.
Transient silencing of target genes was achieved by transfecting the gene-specific siRNAs (Dharmacon, ON-TARGETplus SMARTpool L-014162-00) into 293T cells using Oligofectamine (Invitrogen).
The apparent robustness of Bmp-mediated regulation of Xenopus Vent genes was achieved by co-option of Vent binding sites into their promoters.
Overexpression of each of these genes was achieved by 2μ plasmids previously used by others [69], [70] and verified by real-time PCR (data not shown).
Amplification of the corresponding genes was achieved using specific primers and probe set and measured continuously using an ABI 7900 HTI Detection System.
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Thus, the combinatorial branchial arch expression of Dlx genes is achieved through interactions between signaling pathways and intrinsic cellular factors.
Assessing the proportion of the variation of a trait in a population that is due to genes is achieved by a statistical method called the analysis of variance.
Transmission of genetic information in genes is achieved via complementary base pairing.
Pan-mesodermal expression of muscle-identity genes were achieved with the 24B-Gal4 driver line [36] and the embryos were left to develop at 28°C for 12 hours before fixation.
We chose contrasted culture media to reflect a wide range of possible environmental conditions, starting with 0.25% glucose because from this concentration the maximal expression of glycolytic genes is achieved [52].
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