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Delivering genes to mediate functions of cells is a crucial technology for both basic science and clinical applications.
DNA shuffling utilizes sequence identity between parental genes to mediate template-switching events (the annealing and extension of one parental gene fragment on another) in PCR reassembly reactions to generate crossovers and hence recombination between parental genes.
These findings imply that PHD1KDM5B may help maintain KDM5B at target genes to mediate the demethylation activities of KDM5B.
In fact, chimpanzees also have diversity in bitter taste detection but use different genes to mediate this sensitivity.
The classical view of hormone action is one that depicts ligand-bound AR being recruited to the promoter of target genes to mediate the transcription and translation process.
Besides the SCN, clock genes are also rhythmically expressed in peripheral tissues, suggesting a possible universal role of clock genes to mediate temporal control over physiological diurnal events in peripheral organs.
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We then utilized zinc-finger nucleases (ZFNs), designed to target the endogenous CFTR gene, to mediate correction of the inherited genetic mutation in these patient-derived lines via homology-directed repair (HDR).
FLC would be an obvious candidate gene to mediate this response, however, our studies did not fully support this possibility.
This was shown by construction and analysis of two GFAT deficient strains, E. coli ΔglmS and S. pombe Δgfa1, and the ability of the GFAT encoding gene to mediate plasmid selection.
This was demonstrated through the analysis of two GFAT deficient strains, E. coli ΔglmS and S. pombe Δgfa1, and also the ability of the gene to mediate plasmid selection and stability.
This GO category of genes should be most similar, in terms of function, to genes known to mediate intellectual disability.
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