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The field of synthetic biology involves redesigning networks of genes to enable microbes to perform useful functions efficiently.
A pelB signal peptide was linked to the antibody genes to enable secretion of the expressed polypeptides into the periplasm.
Hypotheses are presented of what could be specified by genes to enable the different functional architectures of the neural networks found in the brain to be built during ontogenesis.
By establishing developmental genetic tools in model systems where sexual selection and conflict are understood, we can begin to reveal how selection can exploit ancient developmental genes to enable the evolution of sexually dimorphic traits.
Over the years the laboratory has developed single gene and gene panel sequencing for a wide range of orphan genetic conditions in addition to performing exon-level array CGH for the detection of intragenic deletions/duplications in these genes to enable comprehensive mutation analysis for these disorders.
These tumor cells have sometimes been labeled with fluorescent [6, 7] or bioluminescent [8, 9] reporter genes to enable specific detection, but the poor penetration of light within the mammalian tissue limits the use of fluorescent and bioluminescent imaging to superficial lesions.
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We have developed a gene disruption cassette, which incorporates the Kanr marker, and direct repeat sequences designed from the target gene to enable the deletion of the gene without the introduction of added DNA sequences.
This plasmid also contains the neomycin-resistance gene to enable selection of permanently transfected clones.
The Ta2776 gene was used as the reference gene [ 68] and was amplified in parallel with the target gene to enable the normalization of gene expression.
An unexplored concept is over-expression of a host essential gene to enable selection in the presence of a chemical inhibitor of the gene product.
This approach could also be used to insert fluorescent or epitope tags into the endogenous copy of any gene, to enable affinity purification or detection of endogenous protein.
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