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The WUCaMP assay uses targeted capture for NGS analysis of 25 cancer-associated genes to detect mutations at actionable loci.
The designed primers were used for the amplification of nifH and nifD genes to detect nitrogenase genes in photosynthetic bacteria.
To determine the role of TFs in this group, we used TFs associated genes to detect the effect of pervasive interactions at Sa and Sb pollen sterility loci (Additional file 8: Figure S5c).
We also calculated Tajima's D values for KPC and Ymf genes to detect selection.
Recent studies have demonstrated the potential power of resequencing candidate genes to detect rare variants underlying disease-associated traits or to identify somatic mutations associated with tumors [11], [12], [13], [14], [15], [16], [17].
Plants use several different types of disease-resistance genes to detect the presence of pathogens and induce defense responses.
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The MSCSA principle was adapted to facilitate the detection of mutations in the UL97 gene to detect ganciclovir resistance of human cytomegalovirus (HCMV) [ 136].
In addition, a V5 epitope-tag was inserted at the carboxyl terminus of gK gene to detect gK.
We used a specific and sensitive PCR assay based on the rfbE gene to detect low levels of these pathogens in wastewater.
We designed six primer pairs, conserved sequences of the variable D1/D2 domains of the 26S rRNA gene, to detect the yeasts and demonstrated their specificity.
Some authors use the 18S or 28S rRNA gene to detect fungal DNA, but the majority of them (including our group) use as target DNA the region between these two genes, ITSs-5.8S rRNA region (Table 2).
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