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"They wanted to create primitive workers from the homo erectus and give him the genes to allow him to think and use tools".
In the present study, we developed a bicistronic commercial vector containing a goat β-casein promoter and an internal ribosome entry site fragment between the human lactoferrin and human lysozyme genes to allow the introduction of both genes into bovine adult fibroblasts in a single transfection.
Gene probes were designed at the 3′ end of the genes to allow detection by hybridization with potentially partial reverse transcriptase products.
He outfitted the sending cell with promoters and genes to allow the controlled expression of a chemical called V. fischeri autoinducer.
Golden rice was first developed in the 1990s as a way to supplement diets lacking in vitamin A. Researchers added genes to allow rice to make beta-carotene, a precursor molecule for vitamin A synthesis.
Besides, the non-recombining Y chromosome harbors too few genes to allow for a valuable comparison with the other chromosomes.
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Primers were designed using conserved DNA regions of the gB gene to allow DNA amplification from all strains of BaCMV.
GAPDH served as the endogenous control gene to allow for normalisation between different RNA samples.
First, TAGIT includes the lacZ gene to allow the rapid identification of in-frame insertions and significantly reduce the number of insertions screened.
The PCR reactions were normalised with L27 (L27 ribosomal gene) to allow for comparison between samples.
All measurements were performed in duplicate and HPRT was used as a housekeeping gene to allow quantification.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com