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In this study, genes shown in previous genomic screens to be required for resistance to gamma radiation in diploid cells were analyzed to identify those genes that specifically affect repair of site-specific DSBs.
Additionally, the comparative RNAseq-based approach revealed a low number of genes significantly regulated in both strains (around 1%) and a low number of genes involved in the non-specific metal response, indicating that the approach was fit for identifying genes that specifically respond to metals.
His lab is identifying novel genes that specifically target neuroblastoma, harnessing information that may be used to create targeted therapies.
We show that two branches of the fly's immunity are important for fighting F. novicida infections in a model host: melanization and an imd-regulated immune response, and identify bacterial genes that specifically counteract these host responses.
The detection of these pathogenic agents in an otherwise healthy plant can be ascribed to the presence and activity of resistance (R) genes that specifically recognize pathogens, which contain complementary avirulence genes (Staskawicz 2001).
This might be important for viral transformation as IFNs are a factor in controlling the transformation process: EBV transformation is inhibited by IFNs and the virus encodes genes that specifically nullify the functions of IFNs [46], [47], [48], [49], [50].
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Antisense oligodeoxynucleotides [ASODNs] are single-strand DNA molecules complementary to regions of a target gene that specifically inhibit gene expression by hybridizing the gene's mRNA [1].
Taking the results from VEGF and AT1R expression analysis into consideration, it was evidenced that it was CD (not p53 gene) that specifically reversed Ang-II-inducible VEGF overexpression by downregulating AT1R expression.
PCR primers (Table 1) were designed using Primer3 [21] for each gene that specifically amplified between 90 120bp.
The same group also reported an estrogen-responsive element in the promoter region of NIS gene that specifically binds ERα and mediates ERα-dependent activation of transcription [18].
b Contains probesets for the IFNγ-Receptor 1 gene that specifically bind the DNA sequence of the gene.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com