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Among all candidate floral homeotic genes tested, only RhAG appeared differentially expressed between 'Malmaison' and 'St Anne's' (Figure 5A).
Of the genes tested, only CCNE1 knockdown showed a significant reduction in cell viability in OVCAR-3 (to approximately 60% of NS control cells; p<0.01; Figure 2C).
Among several candidate target genes tested, only the homeotic gene Abdominal-B was a target of PH in both ovarian and wing disc cells.
Of the five pro-Th17 genes tested, only the TGFβ gene was not upregulated, but, unlike in murine models, the role of TGFβ in Th17 induction in humans is still a contentious issue [31].
There was little evidence that the uterine E. coli isolates possessed the common pathogenicity genes commonly associated with adhesion, invasion and virulence of DEC or ExPEC although the 17 genes tested only represent a small proportion of the available total [14], [26].
Out of the three nitrogen metabolism genes tested, only the glnA-hetR gene pair was co-expressed for all time points measured (Figure 3, Table 4), although they are not closely localized in the Trichodesmium genome (http://genome.jgi-psf.org/finished_microbes/trier/trier.home.html).html
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This task of detecting these genes is a difficult statistical problem; a statistical hypothesis is made for each of tens of thousands of genes tested, but only a small number of replicate arrays are available to test those hypotheses.
As previously mentioned, testing starts in regions most likely to have mutations; other segments of the gene are tested only if negative results are obtained.
Of the 14 gene regions tested, only cox1, cox2, nad2 and nad5 yielded trees similar to Topology 1. Phylogenetic analyses of the atp6, cob, nad4 and nad1 datasets did not allow resolution of the three GFC species (Topology 2), while analyses of the atp8, atp9 and nad4L datasets clustered the GFC species together with F. oxysporum and F. graminearum(Topology 3).
To discover significantly upregulated and downregulated genes between the conditions tested, only the genes with P-values of DESeq analysis less than 0.001 and RPKM values above 20 in at least one condition went into further analysis.
Of the genes that were tested, only SDH1 showed significantly higher expression in o2 at 22 DAP.
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