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Consistent with an enhanced transcription activity, the engineered OCT4 significantly stimulated the expression of genes specifically targeted by OCT4-alone, OCT4/SOX2, and OCT4/SOX2/KLF4 during human iPSC induction, compared with the wt-OCT4.
It is worth noting that in a recent study aimed at identifying host genes specifically targeted by commensal (as opposed to pathogenic) bacteria, IRF9 was one of the most strongly up-regulated host genes by the oral commensal bacterium Streptococcus salivarius K12 [56].
So, how are the cis-element-containing genes specifically targeted by PCFS4?
The functional classes of genes specifically targeted by both genotoxicants were also quite distinct from those targeted by each genotoxicant.
Even though MAM and HN2 both alkylated neuronal DNA, the genes specifically targeted by HN2 were quite distinct from those targeted by MAM.
Much of the work on gene expression has been focused on the role of ERα, but little is known about genes specifically targeted by ERβ or by α/β heterodimers.
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Overall our data indicates that treatment of MCF-7 breast cancer cells with ETP-45658 directs the differential regulation of a specific subset of FOXO target genes, specifically targeting those associated with the cell cycle resulting in the cell cycle arrest response we observe.
Unlike microarrays, which typically measure gene expression in only one or a few regions of a gene specifically targeted by probes, our RNA-Seq method measures strand-specific expression across the entire gene, as can clearly be seen for MMP1 (Figure 3a).
Understanding which genes are specifically targeted by Francisella will further help define the extent and molecular mechanisms of immune suppression.
Thus, we assume that a single gene is specifically targeted in NbMAPKKKβ-silenced NbMAPKKKβ-silenced more highly similar genes might be targeted in NbMAplantssilenced plants.
Nonetheless, this approach requires genes to be specifically targeted, whereas SB transposon mutagenesis is random and may identify genes not otherwise considered.
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