Intra-specific variability across all genes ranged from 41%to250%50 %.
The fraction of this cohort carrying actionable PGx variant(s) in individual genes ranged from 30% (SLCO1B1) to 79% (CYP2D6).
Nucleotide sequence divergence between the coding regions of four genes ranged from 16.0 to 21.6%, and locus-specific eight primer pairs targeting introns were designed.
The amino acid identity of hop RGAs to various R genes ranged from 41% (TIR NBS LRR) to 81% (non-TIR NBS LRR).
The PCR amplification efficiency of these candidate genes ranged from 98.3 to 127.3%, and the regression coefficient (R 2 value) of the standard curve ranged from 0.980 to 0.997, well within the acceptable range of qRT-PCR (Table 1).
For the genotypes with partial aflatoxin gene cluster, the average SNP density for genes ranged from 0 6 SNPs/kb but average SNP density for genotypes ranged from 1 7 SNPs/kb.
In this data platform, the assigned scores for candidate genes ranged from 0 to 4.6.
The level of expression of these genes ranged from 0.02 to 4,704 transcripts per cell.
The number of predicted genes ranged from 2,898 to 3,254, and the average gene length ranged between 845 and 942 base pairs (Table 2).
The effect of the alanine mutation on Adr1-dependent genes ranged from 5-fold or greater decreases in binding and activation (ADH2), to no effect (ADY2).
The number of tRNA genes ranged from 86 to 97.
