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Surprisingly, 1063 genes produced more RNA from 0.5 to 96 hours after death.
We also found that 64% of the alternative spliced genes produced more than two isoforms.
Among the genes that have generated PPs, we found that IL genes produced more PPs than NIL genes.
Furthermore, 44.8% (2,337) of the AS genes produced more than two isoforms (Additional file 14: Table S5).
Comparison of AS events in different tissues revealed that multi-exon genes produced more splice variants in early growth fruits than did in seedlings and flowers.
Other genes produced more ambiguous signals because C. velia fell within a clade of mixed algal types: in 11 trees red and green algae were mixed; in 3 trees red and glaucophyte algae were mixed; and in 5 trees red, glaucophyte, and green algae were mixed (fig. 3 and supplementary fig. S4, Supplementary Material online; table 1).
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Moreover, a combination of several endogenous control genes produces more reliable normalization than any single control gene [ 6].
Thus, the A11 genes produce more proteins than the A12 genes, when mRNA abundance has been controlled for.
Surveys from the human genome estimate that as many as 70% of human genes produce more than one transcribed form [ 1].
In agreement, more than 90% of genes producing more than 16 transcripts were marked by Benzonase and Cyanase hotspots at the TSS; conversely, only 30% of TSSs of inactive genes contained Benzonase-Cyanase hotspots.
From this analysis using known gene models, we found that 1,711 genes among the 8,896 expressed genes produce more than one transcript isoform, among the genotypes examined here (Additional file 11).
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