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Intriguingly, 547 (91%) CD56+ DC highly expressed genes present at higher level in mDCs than pDCs based on cDNA array data (Fig. 3A, right panel; Table S1).
Annotations of candidate genes at the fine-mapped Pup1 locus have not revealed any gene related to mechanisms known to directly affect P uptake or P metabolism (Heuer et al. 2009), and only two of the genes present at the Pup1 locus are present on the Agilent 22 k array.
Of the 14 genes present at high amounts in DGE, nine are short, non-coding RNAs.
Some RNAPII remained bound to the β-major promoter and the promoters of Rad23A and several other genes present at a gene-dense region of mouse chromosome 8, but in each case binding was strongly reduced (Figure 1D,E).
The possibility that the annotated genes were mis-annotated or are pseudogenes and that the nov ORFs are the only genes present at these loci seems unlikely since the majority of the annotated ORFs found opposite the nov genes are well conserved among Pseudomonas spp. or have putative orthologs in other genera (Figure S1).
All genes present at ≥ 1 tpm in either one of the two datasets were enumerated.
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Similarly, 1292 (83%) CD56+ DCs lowly expressed genes presented at lower level in mDCs than pDCs (Fig. 3B, right panel; Table S2).
In total 3 microarrays experiments, genes presented at least twice were expressed, others were not.
All the 50 genes presented at least one interaction in the PPI network produced by the STRING database.
There were multiple alleles of a gene present at different stoichiometric levels due to heteroplasmy in chondriome of each line.
The lowest identity was found for a protein encoded by AFR028W, a gene present at syntenic positions in many yeasts, but with unknown function in S. cerevisiae.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com