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The influenza A virus (H1N1) genome contains 8 genes: polymerase PB2, PB1, PA, hemagglutinin HA, neuraminidase NA, nucleocapsid NP, matrix protein MP and nonstructural gene NS.
However, the 3 polymerase genes (polymerase basic 1, polymerase basic 2, and polymerase acidic) clustered in a different clade.
We would like to see evidence that, at least for some genes, polymerase 2 is active in endothelial cells; the authors only show negative marks (a decrease in permissive histone modifications, with a decrease in RNA polymerase II density).
Phylogenetic trees constructed from the 6 internal genes (polymerase basic [PB] 1 and 2, polymerase acidic, nucleoprotein, matrix [M], and nonstructural [NS]) showed that JXB15, JX77, and JX346 clustered closely with H9N2 viruses isolated from Jiangxi in 2010 and 2011.
To quantitatively estimate the mRNA expressions of several genes, polymerase chain reaction (PCR) amplification was performed on a Light-Cycler™ instrument system (Roche, Mannheim, Germany) using the Light-Cycler-FastStart™ DNA Master SYBR green I kit (Roche).
Sequences derived from most of the other genes (polymerase proteins PB1 and PA, hemagglutinin, nucleoprotein, matrix protein, nonstructural protein) of ORVs and OSVs were phylogenetically interspersed with no distinct clustering.
Similar(52)
Drug-resistant cytomegalovirus causes major problems in immunocompromised patients and is due to mutations in the UL97-gene (phosphotransferase) and/or the UL54-gene (polymerase).
MRSA confirmation was obtained by mecA gene and nuc gene polymerase chain reaction.
Vancomycin resistance was confirmed using vancomycin minimum concentration (MIC) E-test (AB BioDisk, Sweden) and an in-house van gene polymerase chain reaction (PCR).
Clinical isolates were screened using a specific 16S rRNA gene polymerase chain reaction (PCR) assay, and sequenced to confirm taxonomic identities.
After reviewing the literature and the biological activities associated with the EBOV gene 'Polymerase', the researcher would now like to proceed on the next step of his analysis.
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