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With numerous experiments with microliter and nanoliter volumes, Wu et al. [40] concluded that beyond one million reads, the number of detected genes per cell varies less than 5%.
Data generated by droplet-based approaches permit 10,000 s of cells to be counted, while other platforms that use chip-based systems process only 100 s of cells at a time but are more sensitive to calling the number of genes per cell [39].
There were more than 3,000 copy number-altered (CNA) genes per cell line.
Cells bearing ura4 + circles are expected to have multiple ura4 + genes per cell.
For the relative quantification, the copy number of the 16S rRNA genes per cell was averaged to five.
Venn diagrams of the differentially expressed genes per cell line, time point and treatment showing overlap of genes between the different conditions.
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That made the nuclease's task easier, since only one copy of the gene per cell had to be disabled instead of the two copies (one from each parent) that most people have.
The new primer sets allowed detection of the corresponding genotypes in soil with a detection limit of 103 104 or 105 106 gene copies g− 1 soil, assuming one copy of the gene per cell.
SHM results in approximately one nucleotide change per variable gene, per cell division.
One finding from that study is that about 10 13 isoforms are expressed per gene per cell line [ 11].
Fifteen cases (29%) had a maximum of 9 16 copies of the gene per cell (mean=three copies/cell).
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