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The technique was to capture the normal copies of genes made by living cells and to decode a few hundred DNA units from part of the gene.
The lists of the zebrafish chemokine genes made by these two groups also included partial genes.
A two-proportion z-test (see Methods) indicates that there is no significant difference between the two proportions of single exon genes and that there is no bias towards the prediction of single-exon genes made by GreenGenie2.
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First, in 1989 Gross et al. constructed a chimeric TCR (cTCR) gene made by replacing the Vα and Vβ extracellular domains of the TCR chains with their VH and VL immunoglobulin homologs (CαVH + CβVL or CαVL + CβVH).
Clones of hAFSC were transduced with a lentiviral vector (SMPU-R-MNCU3-LUC based on HIV-1 that transduces the firefly luciferase gene) made by the Vector Core Facility at Childrens Hospital Los Angeles following standard protocols as reported in the paper by Crooks et al [29].
If the item is a gene, it reported the prediction of miRNAs targeting such a gene made by TargetScan (59 ), miRanda (61 ) and mirTarBase (60 ).
All the mutations regarding Eastern European population were brought in Table 4. Alu insertion in intronic sequences was detected in Czech population in intron 3 of BRCA1 gene made by AluSx (g.17686 17695) and AluY (g.18760 18769) repeats rearrangements and in intron 10 by means of AluJb (g.33248 33276) sequence recombination.
The prevalent outcome of DNA demethylation is expression of previously silenced genes, made possible by enabling attachment of transcription factors to the genes, with consequent gene transcription and protein synthesis up-regulation [ 49].
Annotations of genes were made by associating human genes with GO terms.
The projections onto transcription factors are constructed by linking every two nodes regulating one common gene at least; similarly, the projections onto regulated genes are made by linking every two regulated genes sharing one common regulator at least.
Deletions of the BSD2 gene, as well as of the ALY1, ALY2, ROD1 and YGR068C genes, were made by replacing the entire coding sequence of the gene with the Schizosaccharomyces pombe HIS5 cassette.
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CEO of Professional Science Editing for Scientists @ prosciediting.com