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For genes lacking a TTHERM_ID in the microarray results, the gene sequence can be retrieved by downloading and searching the cDNA sequence file (TTA1_08302006.cdna) or protein sequence file (TTA1_08302006.pep) from ftp://ftp.tigr.org/pub/data/Eukaryotic_Projects/t_thermophila/annotation_dbs/interim_annotation_release_08313006/.
Genes lacking a Pfam annotation were discarded prior to collation.
Indeed, analysis of mitochondrial genes lacking a poly-A tail demonstrated a large influence of the protocol.
MPSS technology is unable to detect approximately 7% of known genes lacking a DpnII restriction enzyme site [ 30].
We did not make predictions on the singletons or genes lacking a probe on the ATH1 microarray.
However, ~90% of tags identified correspond to genes with associated KOG terms; therefore, many of the genes lacking a KOG term have relatively low expression levels.
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We then asked what which genes lacking an identified role in cardiac development were appropriately expressed in cardiac structures.
All PRR1 homologues had a conserved 6 exon structure and were the only PRR genes lacking an intron in the CCT domain.
Thirteen genes lacking an ortholog were removed.
In mammalian cells, genes containing an intron generally show more expression than the same genes lacking an intron.
Also included in genes lacking an intact homologue in the other two isolates is the strain 10810 locus designated Hib10810.1401.
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