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This study provides new insights into the mechanism by which the post-transcriptional fate of Pol II-transcribed genes is determined.
S. mutans may be identified using simple biochemical tests [5] or with PCR-based methods in which the presence of S. mutans-specific genes is determined [6] [8].
It appears that the number of OR genes is determined primarily by the functional requirement for each species, but once the number reaches the required level, it fluctuates by random duplication and deletion of genes.
Specificity of the SREs in many genes is determined by the base-pair spacing between the half-site repeats, i.e. a spacing of 1, 3, 4 or 5 nucleotides (DR-1, 3, 4 or 5) has been reported to specify a retinoid X responsive element (RXRE), vitamin D response element (VDRE), thyroid hormone response element (TRE) or a retinoic acid responsive element (RARE), respectively [33].
The initial transcription state of all genes is determined by these conditions.
Hence, the direction of the strand asymmetries along genes is determined by the direction of transcription.
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Knock-down efficiency of shRNAs on target genes was determined by qPCR.
Then, DMCs and DVCs within target genes were determined for further analysis.
Expression levels relative to these house-keeping genes were determined by the calculation of ΔCt.
HepG2 cells were treated with GN and the expression of JMJD2B, PPARγ2, and its steatosis genes was determined by qPCR.
mRNA expression levels of indicated genes were determined by quantitative reverse trancriptase PCR in population doublings, PD10 and PD55 IMR90 cells.
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