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We conclude that analyzing 5' capped RNAs in eukaryotic cells and biospecimens aids the definition of the complete Pol II transcriptome and increases the sensitivity of identifying differentially expressed Pol II genes in physiological and pathophysiological states.
Combinatorial binding and control of gene transcription by specific transcription factors allows for individual regulation of each gene and concerted regulation of large sets of genes in physiological and developmental programs.
It will therefore be interesting to monitor the expression of these genes in physiological contexts such as tree defence signaling during biotrophic or necrotrophic pathogen infections, or adaptation to environmental stresses.
Despite many recent advances in functional studies of WRKYs in grapevine, the biological function of most VvWRKY genes in physiological and developmental processes and plant defence still needs to be elucidated.
As CREB/CREM are involved in many physiological and metabolic processes, we used the KEGG pathway function of DAVID annotation (http://david.abcc.ncifcrf.gov/) to make a more comprehensive identification of potential CREB/CREM target genes in physiological functions (see Additional file 9, Table S5).
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We report for the first time an effect of the lack of nm23-1 gene in physiological conditions.
According to slight structural differences with Nlp isoform A, Nlp isoform B might function as a molecular hinge between intracellular trafficking and intraflagellar transport machinery, suggestive of a novel role for NLP gene in physiological photo-reception process and retinal ciliopathies (van Wijk et al, 2009).
Using the human sequence as a reference, the results demonstrate that the missing gene set is enriched for genes implicated in physiological pathways of immunity and organism responses to pathogens (12 genes), olfaction (16) and regulation of transcription (63).
Genes located in physiological trait QTL intervals, showing correlations of the gene expression levels with physiological traits (P < 0.05) are detected by microarray and regression analyses using the subset of F2 mice (n = 16).
All genes involved in drought stress response were classified with respect to genes involved in physiological adaptation and/or molecular adaptation after application of drought stress to the plant.
Functional genetic maps locate genes involved in physiological processes potentially underlying traits and allow identifying functional and positional candidate genes through translational genomics.
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