a, Differentially expressed genes in cell lines that are dependent on GPX4 (left) (n = 113, non-dependent versus dependent, moderated t-statistic FDR estimates).
For example, we can knock out genes in cell cultures and learn more about their function by doing so, or even create strains of harmless bacteria that bio-artists can use to make colorful fluorescent proteins.
We have applied a high-throughput RNA-interference (RNAi) screen of 19,470 double-stranded (ds) RNAs in cultured cells to characterize the function of nearly all (91%) predicted Drosophila genes in cell growth and viability.
We propose that auxin acts independently from and interdependently with PIF and GA pathways to regulate expression of growth-associated genes in cell expansion.
This study illustrates that elevated expression of OsNPF7.2 promotes up-regulation of crucial genes in cell cycle (Fig. 5d), which indicated that over-expression of OsNPF7.2 might promote cell division.
We found that these nine genes are highly predicted to function in the same biological process as cellulose synthase and cellulose synthase-like genes of rice, suggesting a potential role for these nine GH genes in cell wall biosynthesis.
Release of complete genome sequences of several S. zooepidemicus strains and successful development of a markerless gene-deletion system enable us to elucidate the role of individual genes in cell growth and metabolism, which will guide the metabolic engineering of S. zooepidemicus for HA production (Beres et al. 2008; Ma et al. 2011; Sun et al. 2013).
To investigate the role of these two genes in cell metabolism under conditions of stress, we analyzed (1) the structure of the genomic regions flanking the MmTKL1 and MmTKL2 coding regions and (2) the expression profiles and functions of each gene in relation to stress response and erythritol biosynthesis.
The RNA interference pathway is often exploited in experimental biology to study the function of genes in cell culture and in vivo in model organisms.
These data reconfirm the attachment of RbbA and YhjD to the membrane and imply a connection of these two genes in cell division and protein synthesis.
Six of the seven TREs tested using the minimal TATA-box showed various levels of T3-dependent activation of both reporter genes in cell culture.
