Sentence examples for genes driven from from inspiring English sources

T7 RNA polymerase can be induced resulting in expression of genes driven from the T7 lac promoter.

The control bicistronic lentiviral vector RSC-SMPGW has been described previously and contains the P140K version of MGMT for in vivo selection driven from an SFFV promoter, and an EGFP reporter gene driven from the human PGK promoter [14].

To identify novel regulators of SREBPs, we performed a genome-wide cDNA over-expression screen to identify proteins that might modulate the transcription of a luciferase gene driven from an SREBP specific promoter.

To assess if ovine indel SPRN alleles produce stable proteins or proteins with distinct biochemical properties we performed acute transfections into N2a neuroblastoma cells using a bigenic "pBUD" expression vectors encoding Sho alleles of interest plus a GFP reporter gene driven from a separate promoter.

As a negative control, the metK gene driven from its native promoter was also cloned in-frame with the ACD.

Plasmid pCMV-Int harbouring the PhiC31 integrase gene driven from a CMV promoter was a generous gift from Dr. Calos, Stanford University, CA, USA.

Using this method, the gene is disrupted and expression of genes downstream of the disrupted gene is driven from the erythromycin promotor.

The vector used in this trial is based on a SIN γ-retrovirus in which the IL2RG gene is driven from an internal EF1α promoter (see Table 2 for summary).

Previously, we generated a knock-in mouse model of ETV6-RUNX1 ALL, in which expression of the fusion gene is driven from the endogenous Etv6 promoter, and is linked to expression of the Sleeping Beauty (SB) transposase allowing the identification of transposon gene mutations that co-operate with Etv6-RUNX1 in leukemia development [ 7].

YFP- Blm10 was the sole copy of Blm10 in the cell and gene expression was driven from its endogenous promoter in its native chromosomal context.

Clock genes drive both rhythms.