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Gene expression profiling of octanoate-treated hepatocytes shows a pattern of upregulated lipid metabolic genes, demonstrating a specific transcriptional response to lipid.
Genes demonstrating a similarity to the abovementioned insect DNA sequences with an E value < E-03 were discarded.
Values for fold change were calculated and genes demonstrating a net change in gene expression of two fold or greater were selected for further analysis.
Hierarchical clustering of the 874 highly differentially expressed genes demonstrating a statistically significant G × E effect identified strain-specific responses of functional modules to changes in copper.
We identified a specific set of genes demonstrating a high correlation as being differentially expressed during feather and scale development and maturation.
A hierarchical clustering confirmed these findings, showing that MII oocytes and hESC clustered together, sharing a signature of overexpressed genes, demonstrating a close gene expression.
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First, a model including Array as a random factor and Dye, Genotype, Environment, and Genotype by Environment as fixed factors was fit for all genes to identify genes demonstrating an interaction effect between strain and copper environment.
We validated the expression patterns for selected genes, demonstrating an association of almost all most differentially expressed genes with proliferation or cell cycle arrest, consistent with previous senescence studies.
This process is intrinsically linked to the regulation of gene expression, with many genes demonstrating an inverse correlation between the degree of DNA methylation and the level of expression [ 1].
In the inactive state, GAL genes demonstrate a characteristic promoter chromosomal organization; the major upstream activation sequence (UASG) elements lie in open, hypersensitive regions, whereas the TATA and transcription start sites are in nucleosomes.
Thus, the overlapping genes demonstrated a strong tendency to respond to stress predominantly in shoots (Figure 4).
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