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More notably, at 32 °C, the transcript levels of the down-regulated genes at low temperatures in tcd10 mutants were recovered to or even slightly higher than WT plants (Fig. 7a, b, c).
In this study, the tcd10 mutants, under low temperatures (20 °C), had the severe affected transcript-levels of most genes for Chl biosynthesis, photosynthesis and chloroplast development (Fig. 6), whereas the down-regulated genes at low temperatures could be recovered to normal level or even higher WT levels at high temperature (32 °C) (Fig. 7).
As shown in Fig. 1 and Fig. 4, bile and bile acids can induce expression of Vp-PAI genes at low concentration.
It has been shown that both human and mouse pluripotent stem cells promiscuously express many tissue specific genes at low levels [7], [9], [10].
A larger fraction of AS reads cover highly expressed genes, such as those associated with translational processes and housekeeping genes, resulting in relatively lower coverage of genes at low and mid-level abundance.
The practically constant presence of mRNAs of the 5 chestnut genes at low temperature reveals an unknown aspect of clock regulation and suggests a mechanism regulating the transcription of oscillator genes as a whole.
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To conduct the comparative transcriptomic studies on human hepatoma particularly for the genes at low-expression levels, we set out an approach as shown in Figure 1.
We observed that transformed cells were viable when grown at 37°C but not after overnight storage at 4°C, perhaps due to increased sensitivity of cells to leaky expression of the toxic lpa gene at low temperature.
Typically, P. tetraurelia stock 51 expresses the 51H gene at low temperatures.
One mechanism to keep R-gene at low level in absence of pathogen is through miRNA mediated gene silencing [ 32, 33].
It is apparent that most lines have either mutant VHL or express this gene at low levels making any potential correlations between VHL and EGFR problematic.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com