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The growth and development of plants is controlled by specific expression of certain genes at an appropriate time, in a particular tissue, and at appropriate abundance (Wray et al. 2003).
Given an average inter individual variability of 46%, we expect to obtain 162 differentially expressed genes at an EV occurrence≥5/6 and 87 differentially expressed genes at an EV occurrence = 6/6.
For this comparison, we requested at least 2-fold differentially expressed genes at an estimated false discovery rate of ≤0.001 (Table 1).
For such comparison, we requested at least 2-fold differentially expressed genes at an estimated false discovery rate = 0.001 (Table 1).
The identification of stable and reliable human gene to gene coexpression networks is essential to unravel the interactions and functional correlations between human genes at an omic scale.
The web-based POBO program revealed that the invariant core W-box motif (TTGAC) was present in 50/51 genes at an average of 3.73 copies/promoter (t-test p-value <0.0001) while the stringent W-box motif (TTGAC(C/T)) was present in 43/51 genes at an average of 2.12 copies/promoter (t-test p-value <0.0001) (Table S3 (Supporting Information)).
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These results cannot be achieved with a "one-gene-at-a-time" approach.
Microarrays monitor the activity of thousands of genes at a time.
Zinc fingers can also be used for "trait stacking," the positioning of several beneficial genes at a single site.
Most gene therapy techniques use a virus to carry new genes into a cell but cannot direct the virus to insert genes at a specific site.
The technique, which depends on natural agents called zinc fingers, may revive the lagging fortunes of gene therapy because it overcomes the inability to insert new genes at a chosen site.
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