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Trypanosomatids represent a unique case in eukaryotes in that they transcribe protein-coding genes as large polycistronic units, and rarely regulate gene expression at the level of transcription initiation.
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Our mini-gene analysis also rules out the possibility that the 16 hours necessary to transcribe a gene as large as dystrophin could provide enough time for intron 22 removal, even with its slow kinetic of cleavage.
Most of the TPS genes, as well as large numbers of TPS pseudogenes, are found in large genomic clusters which may originate from many gene duplication events leading to the terpene diversity found in the genus.
In normal cells, epigenetic mechanisms such as DNA methylation, histone H3 lysine-27 trimethylation (H3K27me3) and histone H3 lysine-9 trimethylation (H3K9me3) play important roles in silencing individual genes as well as large chromosomal regions or even entire chromosomes such as the inactive X of females (reviewed in ref. 15).
Make sure you keep records of which does are bred by which bucks, and keep rotating the animals to keep the gene pool as large as possible.
This has led to important discoveries, both of the roles of specific genes, as well as larger scale chromosomal copy number changes.
Overall, this study suggests that there are common pathways of epigenetic dysfunction in distinct models transformed by arsenic, cadmium or MNU, which target both individual genes as well as larger chromosomal regions that contain many genes.
ERF subfamily genes were twice as large as the DREB subfamily in Hevea.
There are small copy number variations in normal controls within this interval on chromosome 20, but none overlapping all of these genes or nearly as large as this deletion.
The majority of the pseudogenes are processed and originate from housekeeping genes, with ribosomal protein genes as largest subgroup [ 22, 23].
Indeed, the frequency of repeat elements in t2r gene surrounds is double as large as that observed in v1r gene surrounds.
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