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Through integrating the first two types of data, the correlations between genes are evaluated.
Until reference genes are evaluated on an individual basis for all experimental conditions, the erroneous impact of inappropriate reference gene selection on data interpretation and biological outcome will undoubtedly continue to contribute to inaccurate study conclusions and inconsistencies between reports.
Based on this weighted gene network, the functional distance of any two genes are evaluated using the concept of diffusion distance, and the genes with the k smallest functional distance to gene g are selected as its nearest functional partners (NFPs).
Frequently, several genes are evaluated in parallel and the most stable are selected for further experimentation.
We are well aware that single genes are of little interest in profiling experiments when thousands of genes are evaluated.
During the reuse phase, these genes are evaluated and valued according to the hypothesis contrast described in Section 3.
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Potential off-target changes in other genes were evaluated.
(D,E) The expression of PPARγ2 steatosis target genes was evaluated by qPCR.
(b) The mRNA levels of histone-coding genes were evaluated using RT-qPCR, normalized to Actb expression, and visualized as a heatmap.
The housekeeping genes were evaluated for their expression stability in samples from plants subjected to different forms of abiotic stress.
The expression stability of these candidate reference genes were evaluated using the four methods described below.
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