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These interactions between genes are determined by the network.
When we reach the leaf level of the BDD, the states of all the genes are determined and hence, our seed state for the whole BRN.
The expression levels of the genes are determined by the state variables, which describe states of regulation of the gene expressions.
A cost/benefit analysis of the utility of incorporation of insulators in gene therapy vectors will require further studies in which the effects of insulators on both the therapeutic gene and the flanking genes are determined at a large number of integration sites.
A complete cost/benefit analysis of the utility of incorporation of insulators in gene therapy vectors will require further studies in which the effects of insulators on both the therapeutic gene and the flanking genes are determined at a large number of loci.
First, orthologous genes are determined by sequence similarity, genomic function, and genomic structure information.
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Knock-down efficiency of shRNAs on target genes was determined by qPCR.
Then, DMCs and DVCs within target genes were determined for further analysis.
Expression levels relative to these house-keeping genes were determined by the calculation of ΔCt.
HepG2 cells were treated with GN and the expression of JMJD2B, PPARγ2, and its steatosis genes was determined by qPCR.
mRNA expression levels of indicated genes were determined by quantitative reverse trancriptase PCR in population doublings, PD10 and PD55 IMR90 cells.
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