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Fusion genes are created by genetically fusing the open reading frames of two or more genes in-frame through ligation or overlap extension PCR.
Not all genes are created equal.
Tandemly arrayed genes are thought to be subject to birth-and-death evolution, in which new genes are created by duplication.
The model assumes that new genes are created by repeated gene duplications; and that duplicated genes can be maintained in the genome for a long time, whereas others become deleted or nonfunctional through deleterious mutations [2].
Gene duplication is believed to be the principal cause by which new genes are created.
Hybrid genes are created by trans-splicing, sense/antisense transcription, genome rearrangement, or intergenic splicing between two genes [ 1- 5].
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Using Ingenuity Pathway Analysis IPAA) software, networks for >2 fold down-regulated epigenetic genes and up-regulated autophagy genes were created.
Relative standard curves describing the PCR efficiency of selected genes were created and used to perform efficiency-corrected quantification with the LightCycler Relative Quantification Software (Roche Molecular Biochemicals).
A series of plasmid constructs bearing partial sequences of the viral genes were created and each construct was used as a template for in vitro transcription.
Transgenic plants that expressed artificial microRNAs targeting these two genes were created.
The entire concept of "silent" genes is created by our inability to detect extremely low transcript concentrations.
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