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Therapies based on iPS cells face scrutiny because genes are added to the cells to convert them into stem cells.
The model of the network is in a continuous process of revision and growth as new genes are added and new experimental results become available; see http://www.its.caltech.edu/~mirsky/endomeso.htm (End-mes Gene Network Update) for the latest version.
However, the results show that no new maps or networks become significant for larger sets and moreover, significance of previously identified maps and networks steadily declines as more random genes are added.
Genes are added to the set until a score is maximized.
Thus, if intronless genes are added to the data, only this parameter will be heavily affected.
For instance, if this function is set to one, then individual genes are added to each concatenation step, but if the function is set to ten, then ten genes are added to each concatenation point before a tree is recalculated.
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In comparison with the previous design, new genes were added and others removed, considering the ease of differential diagnosis.
Experts called the work ingenious but pointed out that it had been done in a test tube, to which the RNA corresponding to the disease genes was added.
Because of how the genes were added to the chromosomes, the GM pollen only contains the repressor or the seed-killing genes--but not both.
These three genes were added to the RT-PCR validation set.
Further probes representing 6342 genes were added mainly to allow for data normalization.
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