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The gene copy number of known genes MYCN, DDX1, NAG, MEIS1, TEM8, BPM10, PLEK, PPP3R1 and DNMT3A and anonymous SSH clones g10e3, g9d9, g10d12, g4d5 and g2h10a was determined in 32 other neuroblastoma cell lines with listed primers (Table 1) according to a previously described protocol with BCMA and SDC4 as normalizing control genes and normal human genomic DNA (Roche) as calibrator sample [ 24].
Murine TCC cell line MB49 (Summerhayes and Franks 1979) and human TCC cell line T24 (Bubenik et al. 1973) are well-established; here, MB49 cells transfected with either green fluorescent protein (GFP) or luciferase (luc) reporter genes, and normal T24 cells were cultured in RPMI 1640 medium supplemented with 10 % FBS and pen-strep.
Combined effects of single-gene mutations (G), harvest stage (HS) and sample drying technique (DT) on the proportion of dry matter (DM) and starch degraded ruminally in situ and post-ruminally in vitro were evaluated using four near-isogenic lines in Oh43 inbred background: floury-2 (fl2), opaque-2 (o2), sugary-2 (su2), waxy-1 (wx1) genes and normal Oh43.
The signature consists of over-expressed genes in each subclass (Basal-like: 956 genes, HER2: 125 genes, Luminal A: 387 genes, Luminal B: 291 genes, and Normal breast-like: 134 genes).
Afterwards, the expression levels of miRNA genes and normal genes in the model are set to the values as given by the miRNA-ID and Ensembl-ID.
They also had similar molecular and genetic characteristics with the most frequent rearrangements of Vβ-Jβ1, Jβ2 and Vγ If Vγ10-J regions of TCR genes and normal karyotype.
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Functional work suggests that the p.Ser264Gly mutation may alter splicing of the gene and normal subnuclear localization of CIZ1 protein (Xiao et al., 2012).
There has been one other report of two patients (father and son) with HI due to the R269H mutation in the GLUD1 gene and normal serum ammonia concentrations (35 and 28 μmol/l) (19).
To test this, we conducted a resampling analysis (1000 iterations of 141 randomly sampled genes) and fit normal distributions to the resulting frequency histograms (data not shown).
We observed no difference in the number of genes with cis-SNPs associated with gene expression of the concordantly expressed genes between the leukemia cells (n = 30 genes) and the normal leukocytes (n = 30 genes) (Figure 2a).
By this process, a reduced number of less-atypical genes and of normal genes could be included in a larger CAG.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com