Sentence examples for genes and complementation from inspiring English sources

Systematic deletion mutagenesis of the surE, pcm, nlpD and rpoS genes and complementation studies allowed us to identify the NlpD lipoprotein as the only essential factor for Y. pestis pathogenesis in this locus.

Disruption of multiple genes and complementation of the phenotypes is, however, restricted by the number of available selectable marker genes.

Reporter gene and complementation analyses have previously identified a 2.3 kb region upstream of the INO coding sequence to contain regulatory information sufficient for correct INO expression, termed P-INO [ 11].

Further subcloning showed that growth at 37 °C correlated with an intact RPB1 gene, and complementation tests with the temperature-sensitive allele rpb1-1 (Scafetet al, 1990) firmly established that all four mutants were due to rpb1 mutations.

Genetic manipulations, such as gene deletion, gene complementation, and gene over-expression, are common techniques to investigate fungal gene functions.

The identity of the biosynthetic gene cluster was confirmed by gene inactivation and complementation experiments (the first genetic manipulation of a member of this genus) and a model for abyssomicin C biosynthesis is proposed.

Subsequent gene knockout and complementation methodologies collectively point to one gene with large effect on compensatory performance: GLUCOSE-6-PHOSPHATE DEHYDROGENASE 1 (G6PD1, At5g35790).

The specific involvement of pilus type 2a, but not pilus types 1 and 2b in biofilm development is here confirmed by gene deletion and complementation analysis.

Transgenic plants for 2XFLAG tag, PANS1-GFP-GUS gene fusion and complementation were selected on MS media containing glufosinate ammonium (Sigma) 10 μg/ml with 2%% sucrose.

We recently sequenced and assembled the complete genome of P. lipolytica SCSIO 04301 (Zeng et al. 2014) and have also successfully developed a gene deletion and complementation system in this strain (Wang et al. 2015).

To further investigate the function of protein XF in the pathogenesis of piscine S. agalactiae, the plasmids pSET4s-XF and pSET2-CΔ xf were constructed as xf gene deletion and complementation mutants, respectively.