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For this, the expression plasmid (pKTH5393) containing the L. rhamnosus GG spaFED pilus genes acted as the template for adding the needed nucleotide changes (AAA → GCG for K180A or GAC → GCG for D181A) into the spaD gene by the overlap extension PCR technique67.
A subset of pathway-specific genes acted as efficient predictors of obese or lean class membership when used in Naive Bayes or logistic regression based classifiers.
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It is likely that these genes act as biomarkers for hepatocellular carcinoma therapy.
These observations suggest that the three Runx genes act as functionally redundant oncogenes in T-cell lymphoma development.
In support of this hypothesis, we find that highly pleiotropic genes tend to reside in central positions in protein-protein interaction networks, suggesting that pleiotropic genes act as connecting points between different protein complexes or pathways.
In human development, certain genes act as master switches, ensuring that we are born with similar attributes (one head, two lungs, 10 fingers) in nearly all circumstances.
Notably, the above-mentioned pollen sterility genes act as gametophytic pollen killers, whilst the LTMS gene identified in our study acts as a sporophytic pollen killer.
Many cancer susceptibility genes act as tumor suppressor genes in which inactivation of one allele in the tumor can be detected via loss of heterozygosity (LOH).
In the process of transcriptional regulation, the (output) expression level of regulated genes acts as a sensor for the (input) concentration of transcription factors.
This unexpected result supports the view that an important part of the modulated genes act as upstream or downstream effectors of STAT5.
Tumor suppressor genes act as recessive determinants of cancer.
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