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The purified DNA was used for HLA-DRB1 and HLA-DQB1 determination with the PCR-based Micro-SSP DRB and DQB generic typing trays (Biotest AG, Dreiech, Germany).
HLA-DRB1 generic typing was performed by PCR-SSOP (sequence-specific oligonucleotide probes) reverse hybridization using the Luminex technology after locus-specific exon 2 amplification.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com